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  • 04-142 - Anti-IRF4 Antibody, clone 3E4

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04-142 Sigma-Aldrich

Anti-IRF4 Antibody, clone 3E4

clone 3E4, from rat

Synonym: Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, LSIRF, NF-EM5, PU.1 interaction partner, Transcriptional activator PIP

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IQ-IZ, Primary Antibodies
clone   3E4, monoclonal
biological source   rat
application(s)   flow cytometry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, human, human, human
species reactivity (predicted by homology)   mouse (based on 100% sequence homology), mouse (100% sequence homology), mouse (based on 100% sequence homology)
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_038702
UniProt accession no.   Q64287

Description

General description

Interferon regulatory factor 4, also known as IRF4, belongs to the IRF family. IRF4 encodes a 450 amino acid polypeptide with a predicted molecular weight of 51.6 kDa (Grossman 1996). The IRF4 gene is expressed in the spleen, lymphocytes and melanocytes, and is induced in to T cells after cross linking with the T cell receptor. IRF4 is a transcriptional activator that binds to the interferon stimulated response element (ISRE) of the MHC class I promoter. IRF4 is essential for mature T and B lymphocyte function. IRF4 interacts with MYD88 to prevent the interaction of IRF5, resulting in negative regulation of Toll Like Receptor (TLR) signaling. High levels of IRF4 have been associated with Epstein Barr Virus (EBV) and may be critical to EBV transformation. Further oncogenic potential has been associated with the involvement of IRF4 with hair color and pigmentation of the skin, which may cause multiple myeloma.

Immunogen

GST-tagged recombinant protein corresponding to the C-terminus of mouse IRF4.

Application

Anti-IRF4 Antibody, clone 3E4 is a highly specific rat monoclonal antibody, that targets IRF4 & has been tested in western blotting, IHC & Flow Cytometry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected IRF4 in positive human tonsil tissues.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected IRF4 in wildtype and IRF4 knockout mice (Zheng, Y., et al. (2009). Nature. 458(7236):351-356.).

Research Category
Inflammation & Immunology

Research Sub Category
Transcription Factors

Target description

~51 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected IRF4 in 10 µg of Ramos cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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