• USA Home
  • 04-1467 - Anti-PABP 1 Antibody, clone 10E10

EMAIL THIS PAGE TO A FRIEND
04-1467 Sigma-Aldrich

Anti-PABP 1 Antibody, clone 10E10

clone 10E10, from mouse

Synonym: PABP 1, Poly(A)-binding protein 1, poly(A) binding protein, cytoplasmic 1, poly(A) binding protein, cytoplasmic 2, poly(A)-binding protein, cytoplasmic 2

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, P-PA, Primary Antibodies
clone   10E10, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_002559.2
UniProt accession no.   P11940

Description

General description

Poly(A)-binding protein (PABP 1) is an abundant protein that interacts with the 3′ poly(A) tail of most eukaryotic mRNAs. In vivo this protein is involved in many aspects of mRNA metabolism, including mRNA stability, translationally coupled mRNA turnover, protein biosynthesis, and ribosome biogenesis. PABP1 shuttles between the cytoplasm and the nucleus and may bind to cytoplasmic RNA sequences other than poly(A). PABP1 accumulates in the nucleus if transcription is inhibited, suggesting that nuclear export is coupled to active transcription.

Specificity

This antibody recognizes PABP 1.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human PABP 1.

Application

Anti-PABP 1, clone 10E10 is a Mouse Monoclonal Antibody for detection of PABP 1 also known as PABP 1, Poly(A)-binding protein 1, poly(A) binding protein cytoplasmic 1 & has been validated in WB, ICC & IP.

Immunoprecipitation:
Cited in Thoma, C., et al. (2008) RNA. 14:1579-1589, and Buchet-Poyau, K., et al.(2007) Nuc. Acids Res. 35:1289-1300 using a representative lot.

Immunocytochemistry:
Cited in Afonina, E, et al. (1998) J. Biol. Chem., 273: 13015-21 (1998) using a representative lot.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Target description

~ 71 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected PABP 1 in 10 µg of HeLa cell lysate.

Linkage

Replaces: 05-847

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?