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04-1530 Sigma-Aldrich

Anti-MDM2 Antibody, clone 3G9

Anti-MDM2 Antibody, clone 3G9 is a Mouse Monoclonal Antibody for detection of MDM2 also known as Double minute 2 protein or Mdm2 p53 binding protein homolog & has been validated in WB, IP, ICC & IHC.

Synonym: Double minute 2 protein, Mdm2 p53 binding protein homolog (mouse), Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, Oncoprotein Mdm2, double minute 2, human homolog of, mouse double minute 2 homolog, mouse double minute 2, human homolog of, p53-binding protein Mdm2, p53-binding protein, p53-binding protein, ubiquitin-protein ligase E3 Mdm2

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Purified
control   MCF-7 cell lysate
molecular weight   ~ 75 kDa
application(s)   Western Blotting
  Immunoprecipitation
  Immunocytochemistry
  Immunohistochemistry
clone   Monoclonal Antibody
  3G9
purification method   Protein G Purified
epitope   Unknown
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Mouse
species reactivity   Human, Mouse
NCBI accession no.   NP_002383.2
UniProt accession no.   Q00987
gene symbol   MDM2(4193)
  HDM2
  HDMX
material size   100 µg
shipped in   wet ice
storage conditions   Stable for 1 year at 2-8°C from date of receipt.

Description

Background information

MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumor suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.

Physical form

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Application

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)

Specificity

This antibody recognizes MDM2.

Immunogen

His-tagged recombinant protein corresponding to human MDM2.

Species Reactivity Notes

Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.

Quality Assurance

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM2 on 10 µg of MCF-7 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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