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  • 04-796 - Anti-Akt1/PKBα Antibody, clone AW24 | 04-796

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04-796 Sigma-Aldrich

Anti-Akt1/PKBα Antibody, clone AW24 | 04-796

clone AW24, from rabbit

Synonym: AKT1 kinase, Protein kinase B, RAC-alpha serine/threonine-protein kinase, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AE-AK, Alphabetical Index, Antibodies, Primary Antibodies
clone   AW24, monoclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   rat, human, mouse
shipped in   dry ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_150233.1
UniProt accession no.   P31749

Description

General description

The serine threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

Specificity

This antibody recognizes Akt1/PKBα.

Immunogen

Epitope: C-terminus

Sythetic peptide (C-RPHFPQFSYSASGTA) corresponding to the C-terminal (residues 466-480) of rat Akt1/PKBα.

Application

Anti-Akt1 Antibody, clone AW24 detects level of Akt1/PKBα & has been published & validated for use in WB & IP.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Target description

~60 kDa

Physical form

Cultured supernantant containing 0.05% sodium azide.

Format: Purified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by western blot on RIPA lysates from human HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Akt1/PKBα in RIPA lysates from human HEK293 cells.

Linkage

Replaces: 05-796

Analysis Note

Control
HEK293 cell lysate.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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