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  • 04-816-I - Anti-phospho-PPARgamma (Ser112) Antibody, clone AW504 rabbit monoclonal

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04-816-I Sigma-Aldrich

Anti-phospho-PPARgamma (Ser112) Antibody, clone AW504 rabbit monoclonal

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Synonym: Nuclear receptor subfamily 1 group C member 3, Peroxisome proliferator-activated receptor gamma

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Properties

clone   AW504, monoclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   mouse
shipped in   dry ice
isotype   IgG
UniProt accession no.   P37231
Gene Information   mouse ... PPARG(19016)

Description

General description

Peroxisome proliferator-activated receptor gamma (UniProt: P37231; also known as PPAR-gamma, Nuclear receptor subfamily 1 group C member 3) is encoded by the PPARG (also known as NR1C3) gene (Gene ID: 5468) in human. Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor family of transcription factors that mediate a variety of cellular processes, including glucose and lipid metabolism, inflammatory responses, and regulation of apoptotic cell death. They act by binding to specific peroxisome proliferator-response elements (PPREs) on target genes. Three types of PPARs exist, namely alpha, delta, and gamma. In addition, alternative splicing produces three PPAR-gamma isoforms. All PPARs heterodimerize with retinoid X receptor (RXR) and bind PPREs in the promoter region of target genes. Highest expression of PPAR gamma is found in the adipose tissue and lower levels are detected in skeletal muscle, spleen, heart and liver. It plays a critical role in adipocyte differentiation and fat deposition. PPAR-gamma is phosphorylated in basal conditions and is dephosphorylated upon treatment with ligands. Its dephosphorylation at serine 112 induces its adipogenic activity. PPAR-gamma can be activated by prostaglandin PGJ2 and certain members of the 5-HETE family of arachidonic acid metabolites, including 5-oxo-15(S)-HETE and 5-oxo-ETE.

Specificity

Clone AW504 is a rabbit monoclonal antibody that specifically detects PPAR gamma phosphorylated on serine 112.

Immunogen

KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phosphoserine 112 from human PPAR gamma, isoform 2.

Application

Anti-phospho-PPARgamma (Ser112), clone AW504 rabbit monoclonal, Cat. No. 04-816-I, is a highly specific rabbit monoclonal antibody that targets Peroxisome proliferator-activated receptor gamma and has been tested for use in Western Blotting.

Target description

∼55 and 58 kDa observed; 57.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Rabbit monoclonal antibody in cell culture supernatant with 0.05% sodium azide.

Format: Culture Supernatant

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in NIH3T3/L1 cells differentiated into adipocyte-like cells.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-PPARgamma (Ser112) in NIH3T3/L1 cells differentiated into adipocyte-like cells.

Other Notes

Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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