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  • 05-1106 - Anti-IGF-IR Antibody, β-subunit, clone 1-2

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05-1106 Sigma-Aldrich

Anti-IGF-IR Antibody, β-subunit, clone 1-2

clone 1-2, from mouse, purified by affinity chromatography

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, I-IG, Primary Antibodies
clone   1-2, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   rat, human, mouse, mouse, rat
species reactivity (predicted by homology)   human
shipped in   wet ice
isotype   IgG2b
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NM_000875.3
UniProt accession no.   P08069
Gene Information   human ... IGF1R(3480)

Description

General description

IGF-I and -II signal through the IGF-I Receptor, which is homologous to the Insulin Receptor. The high-affinity IGF-II Receptor does not play a direct role in signaling, but regulates the concentration of free IGF-II. The IGFs are involved in skeletal growth, and are essential for prevention of apoptosis. Serum levels of free IGFs are kept low by the action of IGF binding proteins (IGFBPs), which sequester the IGFs. Overexpression of IGFBPs may induce apoptosis, presumably by reduction of free IGF; IGFBP levels are also altered in some cancers. The IGF-I Receptor is not as mitogenic as some other growth factor receptors, but its ability to activate the PI3 Kinase pathway, through the Insulin Receptor Substrate (IRS) proteins, is critical for mediating cell survival.

Specificity

Recognizes the β-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 1323-1337 of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the α- and β-subunits, which are disulfide-linked in a β-α-α-β configuration in the mature receptor. The α-subunit is completely extracellular, while the β-subunit spans membrane.

Immunogen

A 15-mer peptide corresponding to a.a. 1323-1337 (Tyr-RKNERALPLPQSSTC) from the C-terminal of β-subunit of human IGF-1R.

Epitope: Between a.a. 1323-1337 of IGF-1R

Application

Anti-IGF-IR Antibody, β-subunit, clone 1-2 is an antibody against IGF-IR for use in WB, IH(P).

Immunohistochemistry(paraffin): Representative images from a previous lot.
Optimal Staining of IGF Monoclonal Antibody: Placenta

Western Blot: A previous lot of this antibody was used at 1-2 µg/mL for 2 hours at room temperature.

Optimal working dilutions must be determined by end user.

Research Category
Signaling

Apoptosis & Cancer

Research Sub Category
Insulin/Energy Signaling

Target description

Recognizes the beta-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R; ~140 kDa)

Physical form

Affinity Purfied

Purified mouse monoclonal IgG2b in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Storage and Stability

Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on C2C12 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected IGF-1 Receptor beta on 10 μg of C2C12 lysates.

Linkage

Replaces: MAB1123

Analysis Note

Control
C2C12 Cell Lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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