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  • 05-1526 - Anti-Importin subunit alpha-1 Antibody, clone 8G5

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05-1526 Sigma-Aldrich

Anti-Importin subunit alpha-1 Antibody, clone 8G5

clone 8G5, from mouse

Synonym: karyopherin alpha 1 (importin alpha 5), importin alpha 5, importin-alpha-S1, recombination activating gene cohort 2, Karyopherin subunit alpha-1, Nucleoprotein interactor 1, RAG cohort protein 2, karyopherin alpha 1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IH-IM, Primary Antibodies
clone   8G5, monoclonal
biological source   mouse
application(s)   western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_002255.3
UniProt accession no.   P52294
Gene Information   human ... KPNA1(3836)

Description

General description

The importin alpha/beta heterodimer facilitates the translocation of hundreds of proteins across the nuclear envelope. Importin alpha binds to proteins containing a classical nuclear localization signal and links them to importin beta ((KPNB1), a protein which ferries the complex through the nuclear pore complex. Movement through the nuclear pore is mediated by the small GTP binding protein Ran via an energy requiring mechanism. Using nucleotide triphosphates and Ran, the nuclear transport complex moves into the nuclear pore and the importin subunits dissociate. Importin alpha and its passenger protein enter the nucleoplasm while importin beta remains at the pore.

Specificity

This antibody recognizes Importin subunit alpha-1.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human Importin subunit alpha-1.

Application

Anti-Importin subunit alpha-1 Antibody, clone 8G5 is a mouse monoclonal antibody for detection of Importin subunit alpha-1 also known as karyopherin alpha 1, importin alpha 5, importin-alpha-S1 & has been validated in WB.

Research Category
Protein Trafficking

Research Sub Category
Chaperones

Target description

~ 60 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.01 µg/ml of this antibody detected Importin subunit alpha-1 on 10 µg of K562 cell lysate.

Analysis Note

Control
K562 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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