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  • 05-1573 - Anti-Pax5 Antibody, clone 1H9

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05-1573 Sigma-Aldrich

Anti-Pax5 Antibody, clone 1H9

Detect PAX5 using this rat monoclonal antibody, Anti-Pax5 Antibody, clone 1H9 validated for use in western blotting & IHC.

Synonym: Paired box protein Pax-5, B-cell-specific transcription factor, BSAP

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, P-PA, Primary Antibodies
clone   1H9, monoclonal
biological source   rat
application(s)   immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, human
shipped in   wet ice
isotype   IgG2aκ
antibody product type   primary antibodies
NCBI accession no.   NP_032808
UniProt accession no.   Q02650

Description

General description

Pax5, also known as B-cell-specific transcription factor (BASP), and encoded by the gene Pax5/Pax-5, plays a role in B cell differentiation and neural development and spermatogenesis. Pax5 regulates the expression of CD19 a B lymphoid specific target gene. Additionally, Pax5 interacts with ETS1 and alters its DNA binding abilities and it interacts with DAXX and TLE4. Pax5 is localized to the nucleus. Pax5 is expressed at early B-cell differentiation and in the developing CNS during development. In the adult it is expressed in the testis. Pax5 is also involved in B-cell related diseases and lymphomas and most recently was shown to be the controlling factor of the “Z” protein BZLF1 which controls the latent to lytic switch of Epstein-Barr virus (EBV) in humans.

Immunogen

GST-tagged recombinant protein corresponding to mouse Pax5.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pax5 in 10 µg of mouse E13-E14 brain tissue lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Isocitrate dehydrogenase in human tonsil and mouse hindbrain tissue.

Target description

~42 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pax5 in 10 µg of Ramos cell lysate.

Linkage

Replaces: MABE517

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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