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  • 05-166 - Anti-IGF-II Antibody, clone S1F2

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05-166 Sigma-Aldrich

Anti-IGF-II Antibody, clone S1F2

Detect IGF-II using this Anti-IGF-II Antibody, clone S1F2 validated for use in ELISA, IP, IC, NEUT & WB.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, I-IG, Primary Antibodies
clone   S1F2, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  neutralization: suitable
  western blot: suitable
species reactivity   rat, human, human, rat
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_000612
UniProt accession no.   P01344
Gene Information   human ... IGF2(3481)

Description

General description

Human Insulin-like Growth Factor II (IGFII) contains 67 amino acids and shares similar structural features with IGFI, including a 62% sequence homology. In human plasma, IGFI and IGFII are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGFII binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGFII is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells.

Specificity

Less than 10% cross-reactivity to human IGF-I (Tanaka, H, 1989; Suzuki, T, 1989).

This antibody detects IGF-II.

Immunogen

Partially purified rat IGF-II. Clone S1F2

Application

Neutralization: 10 μg/mL of a previous lot inhibited the stimulatory effects of IGF-II (10 ng/mL) on DNA synthesis in chicken embryo fibroblasts. Cell viability was assessed using an ATP endpoint assay (ATPLiteÔ-M, Packard Instruments). Previous lots of antibody were tested to inhibit IGF-II-dependent incorporation of [3H]-thymidine

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Target description

~7 kDa

Physical form

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing PBS, pH 7.4., purified by ammonium sulfate precipitation and DEAE-cellulose chromatography. Frozen solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting on HeLa acid extracted nuclear preps (positive) and recombinant Histone H3 (negative).

Western Blotting Analysis:
1:5,000 – 1:10,000 dilution of this antibody was used to detect trimethylated-Histone H3 (Lys4) in HeLa nuclear acid extracts.

Analysis Note

Control
Human head and neck tumours, angioma tissues

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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