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  • 05-182 - Anti-FAK Antibody, clone 2A7

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05-182 Sigma-Aldrich

Anti-FAK Antibody, clone 2A7

clone 2A7, Upstate®, from mouse

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, F-FA, Primary Antibodies
clone   2A7, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
species reactivity   avian, rat, human, mouse
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_005607.3
  NM_153831.2
UniProt accession no.   Q05397
Gene Information   human ... PTK2(5747)

Description

Specificity

Recognizes FAK.

Immunogen

Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing p125FAK.

Application

Anti-FAK Antibody, clone 2A7 detects level of FAK & has been published & validated for use in IC & IP.

Not recommended in Western Blot

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Target description

125 kDa

Physical form

Format: Purified

Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in Purified immunoglobulin in 10mM PBS, pH 7.4 containing no preservatives.

Protein G Purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

routinely evaluated by immunoprecipitation of FAK from a mouse 3T3/A31 RIPA cell lysate, subsequently immunobloted with Anti-FAK (Catalog #06-543)

Linkage

Replaces: 04-591

Analysis Note

Control
Expressed in most tissue, 3T3/A31 cell lysate

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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