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05-184 Sigma-Aldrich

Anti-Src Antibody, clone GD11

Anti-Src Antibody, clone GD11 detects level of Src & has been published & validated for use in IP & WB.

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (05-184-AF488)
  200 µg (05-184-AF488)
isotype   IgG1
gene symbol   SRC(6714)
trade name   Upstate
brand family   Upstate
format   Purified
molecular weight   60 kDa
application(s)   Immunoprecipitation
  Western Blotting
clone   Monoclonal Antibody
  GD11
host   Mouse
species reactivity   Avian
  Human
  Mink
  Mouse
  Rat
NCBI accession no.   NM_005417.3
  NM_198291.1
UniProt accession no.   P12931
gene symbol   ASV
  SRC1
  c-SRC
  c-src
  p60-Src
  pp60c-src
shipped in   dry ice
Quality Assurance   routinely evaluated on RIPA lysates from non-stimulated A431 carcinoma cells, RIPA lysates from EGF-stimulated A431 cells, mouse 3T3/A31 cells or Cos-1 cells transfected with pUSE Mouse src

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Positive Control Included: Non-Stim A431 Cell Lysate (12-301). Chicken Embryo Fibroblast (CEF) cells expressing Src protein and plated on fibronectin or Non-stimulated human A431 cell lysate

Application

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

General description

Src is a family of proto-oncogenic tyrosine kinases originally discovered by J. Michael Bishop and Harold E. Varmus. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry.
Some tumor-inducing retroviruses (such as Rous sarcoma virus (RSV)), contain a gene called v-src (viral-sarcoma). It was found that the v-src gene in RSV is required for the formation of cancer.
Src tyrosine kinases transmit integrin-dependent signals central to cell movement and proliferation. Hallmarks of v-src induced transformation are rounding of the cell and the formation of actin rich podosomes on the basal surface of the cell. These structures are correlated with increased invasiveness, a process thought to be essential for metastasis.
v-src lacks the C-terminal inhibitory phosphorylation site, and is therefore constitutively active as opposed to normal src (c-src) which is only activated under certain circumstances where it is required (e.g. growth factor signaling). v-src is therefore an instructive example of an oncogene whereas c-src is a proto-oncogene.

Immunogen

Epitope: amino acids 82-169

Recombinant chicken pp60src; v-src

Specificity

Recognizes pp60src.

Physical form

Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Protein G Purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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