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05-201 Sigma-Aldrich

Anti-Fas Antibody (human, activating), clone CH11

Detect Fas using this Anti-Fas Antibody (human, activating), clone CH11 has been published and validated for use in Flow Cytometry (FC), Immunocytochemistry (IC) and Western Blot (WB).

Synonym: APO-1 cell surface antigen, CD95 antigen, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily, member 6

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   50 µg (05-201)
availability   not available in
  not available in Japan
isotype   IgM
gene symbol   FAIM2(23017)
  FAS(355)
trade name   Upstate
brand family   Upstate
format   Affinity Purified
molecular weight   43 kDa
application(s)   Flow Cytometry
  Immunocytochemistry
  Western Blotting
clone   Monoclonal Antibody
  CH11
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Mouse
species reactivity   Human
NCBI accession no.   NM_152877
UniProt accession no.   P25445
gene symbol   ALPS1A
  APO-1
  APT1
  CD95
  FAS1
  FASTM
  LFG
  NMP35
  OTTHUMP00000059646
  TNFRSF6
shipped in   dry ice
Quality Assurance   Routinely evaluated by demonstrating cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.

Apoptosis Assay Analysis:
15-20 µg/mL of this lot maximally induced apoptosis of human Jurkat cells with 83% mortality after 24 hours of treatment.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Human liver tumor, human breast tumor or Jurkat whole cell lysate, Raji cell lysate.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blot:
0.5-2 μg/mL of a previous lot detected Fas in a Raji cell lysate.
Immunocytochemistry:
5-10 μg/mL of a previous lot detected Fas on HeLa cells fixed with 4% formalin/2% acetic acid.
Flow cytometry:
A previous lot of was tested by an independent laboratory using 20 μg/mL of anti-Fas, clone CH11 (Yonehara, S., 1989; Kobayashi, N., 1990).

General description

Fas/APO-1/CD95 (36 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily, a family of transmembrane receptors. Fas has been shown to be an important mediator of apoptotic cell death, as well as being involved in inflammation. Binding of the Fas ligand (Fas-L) induces trimerization of Fas in the target cell membrane. Activation of Fas causes the recruitment of Fas-associated protein with death domain (FADD) via interactions between the death domains of Fas and FADD. Procaspase 8 binds to Fas-bound FADD via interactions between the death effector domains (DED) of FADD and pro-caspase 8 leading to the activation of caspase 8. Activated caspase 8 cleaves (activates) other procaspases, in effect beginning a caspase cascade that ultimately leads to apoptosis. Caspases cleave nuclear lamins, causing the nucleus to break down and lose its normal structure. Fas-induced apoptosis can be effectively blocked at several stages by either FLICE-inhibitory protein (FLIP), by Bcl-2, or by the cytokine response modifier A (CrmA).

Biological Activity
The antibody demonstrates cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.

Immunogen

FS-7 (human diploid fibroblast cell line). Clone CH-11.

Specificity

This antibody recognizes the human cell surface antigen Fas, Mr 43 kDa expressed in various human cells, including myeloid cells, T lympho-blastoid cells, and diploid fibroblasts.

Physical form

Immunoaffinity Chromatography

Purified mouse monoclonal IgM in buffer containing PBS, pH 7.2, with 50% glycerol. Liquid at -20ºC.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial prior to removing the cap.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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