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  • 05-338 - Anti-Fas Antibody (human, neutralizing), clone ZB4

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05-338 Sigma-Aldrich

Anti-Fas Antibody (human, neutralizing), clone ZB4

clone ZB4, Upstate®, from mouse

Synonym: APO-1 cell surface antigen, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, CD95 antigen, FASLG receptor, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6, tu

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, F-FA, Primary Antibodies
clone   ZB4, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  neutralization: suitable
  western blot: suitable
species reactivity   human, human
shipped in   dry ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_152877
UniProt accession no.   P25445

Description

General description

Fas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.

This product does not induce apoptosis in cell culture.

Specificity

This antibody does not recognize TNF and mouse Fas.

This antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.

Immunogen

Recombinant human Fas.

Application

Anti-Fas Antibody (human, neutralizing), clone ZB4 detects level of Fas & has been published & validated for use in WB, FC, NEUT.

Flow Cytometry: A previous lot of this antibody used 5-20 μg/mL. Western Blot: A previous lot of this antibody used 5 μg/mL.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Target description

45 kDa

Physical form

Ammonium sulfate and purified mouse monoclonal IgG1 in PBS containing 50% glycerol. Store at -20°C.

Format: Purified

Protein A Purfied

Storage and Stability

Stable for 1 year at from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by neutralization of apoptosis induced by anti-Fas (human, activating), clone CH11 (Catalog # 05-201) treatment of human Jurkat cells.

Neutralization: 10-500 ng/mL of this antibody was incubated for one hour with human Fas-transfected cells and inhibited the anti-Fas (human, activating), clone CH11 (Catalog # 05-201). Cell viability was assessed using a WST-1 assay

Analysis Note

Control
Various human cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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