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05-369 Sigma-Aldrich

Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6

Detect Na+/K+ ATPase α-1 (sodium/potassium ATPase subunit alpha 1) using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.

Synonym: ATPase, Na+/K+ transporting, alpha 3 polypeptide, Na(+)/K(+) ATPase alpha(III) subunit, Na(+)/K(+) ATPase alpha-3 subunit, Na+/K+ -ATPase alpha 3 subunit, Na+/K+ ATPase 3, Sodium pump subunit alpha-3, dystonia 12, sodium pump 3, sodium-potassium-ATPase,

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   25 μg (05-369-25UG)
  50 µg (05-369-25UG)
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
biological source   mouse
clone   C464.6, monoclonal
isotype   IgG1κ
shipped in   dry ice
species reactivity   mouse, Xenopus, sheep, canine, rat, human, monkey, pig, rabbit
gene symbol   ATP1A3(478)
trade name   Upstate
brand family   Upstate
format   Purified
molecular weight   112 kDa
clone   Monoclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
gene symbol   DYT12
  EC 3.6.3.9
  MGC13276
  RDP
Quality Assurance   Routinely evaluated in western blot on rat brain microsomal preparations.

Western Blot Analysis:
0.05-0.1 µg/mL of this lot detected the α-1 subunit in 20 µg of rat brain microsomal preparation (Catalog # 12-144). To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

112 kDa

Analysis Note

Control
Alpha 1 mRNA is present in all cell types examined.

Application

Immunoprecipitation:
2 µg of a previous lot immuno-precipitated the α-1 subunit from rat brain microsomal preparations.

Immunohistochemistry:
1-10 µg/mL of a previous lot of this antibody was used in immunohistochemistry on ice-cold methanol-fixed tissue.

1. Do not boil microsomes. Warming to 37ºC for 15 minutes is recommended.
2. Microsomes from some tissues may contain sodium pump subunits which are below the level of detection by this antibody.
3. Microsomes derived from tissues which are more difficult to prepare, such as smooth and skeletal muscle, frequently show non-specific cross-reactivity.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

General description

Na+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume.
In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.

Immunogen

Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

This antibody recognizes the α-1 subunit isoform of Na+/K+ ATPase.

Physical form

Format: Purified

Protein G Chromatography

Purified mouse monoclonal IgG1κ in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at
-20ºC.
Produced from a culture supernatant of a hybridoma produced by fusing Ag8 mouse myeloma cells with immunized Balb/c splenocytes. Clone C464.6 also called 6H.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Making Headway
Protocols & Articles
Peer-Reviewed Papers
15

References

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