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05-432 Sigma-Aldrich

Anti-NR1 Antibody, CT

Anti-NR1 Antibody, CT detects level of NR1 & has been published & validated for use in IC, IP & WB.

Synonym: Grin1, NMDA R1 receptor C1 cassette, glutamate receptor, ionotropic, N-methyl D-aspartate 1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   200 µg (05-432)
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
biological source   mouse
clone   monoclonal
isotype   IgG
shipped in   wet ice
species reactivity   , rat, human, mouse
trade name   Upstate
brand family   Upstate
format   Purified
molecular weight   130 kDa observed
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
Species Reactivity Notes   Rat. Immunoreactivity is predicted for human and mouse.
gene symbol   Grin1
  NR1
  Nmdar1
Quality Assurance   Routinely evaluated by Western Blot on rat brain microsomal protein preparation.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected NR1 from 20 μg of rat brain microsomal protein preparation (Catalog # 12-144).

Description

Target description

130 kDa observed

Application

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated NR1 from 500mg of rat brain microsomal protein preparation.

Immunocytochemistry:
An independent laboratory has shown positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.

Note: Do not boil the microsomal preparation. Incubate at room temperature for 30-45 minutes.

General description

NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteriztic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

Immunogen

Epitope: The epitope maps to a region (a.a. 834-864) common to all NR1 isoforms

His-tag fusion protein corresponding to residues 834-938 of rat NR1A. The immunizing sequence is identical in rat and human.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Specificity

This antibody is specific for NR1, Mr 130 kDa. The epitope maps to a region (834-864) common to all NR1 isoforms and so should cross react with all NR1 isoforms. An additional band may be detected at higher concentrations, Mr 200kDa.

Physical form

Format: Purified

Purified mouse monoclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2° to 8ºC from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Making Headway
Protocols & Articles
Peer-Reviewed Papers
15

References

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