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  • 05-511 - Anti-TCF-4 Antibody, clone 6H5-3

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05-511 Sigma-Aldrich

Anti-TCF-4 Antibody, clone 6H5-3

Use Anti-TCF-4 Antibody, clone 6H5-3 (Mouse Monoclonal Antibody) validated in EMSA, ICC, IHC, IP, WB to detect TCF-4 also known as Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2.

Synonym: HMG box transcription factor 4, Transcription factor 7-like 2, T-Cell factor 4, T-cell-specific, HMG box, T-Cell-specific transcription factor 4, Transcription factor 7-like 2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TC-TE
clone   6H5-3, monoclonal
biological source   mouse
application(s)   electrophoretic mobility shift assay: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, human
shipped in   wet ice
isotype   IgG2a
antibody product type   primary antibodies
mfr. no.   Upstate®
GenBank® accession no.   CAB97213.1
UniProt accession no.   Q9NQB0
Gene Information   human ... TCF7L2(6934)

Description

General description

TCF4, also known as TCF7L2, is expressed widely during development. Gene targeting study indicates that it is required to maintain the crypt stem cells of the small intestine. TCF4 has many different splicing isoforms and they are expressed differentially in tissues and in cancers of different stages. Studies also indicate that variant of the TCF4 gene confers an increased risk of type 2 diabetes.

Specificity

This antibody recognizes TCF-4

Immunogen

Amino acids 31-331 of human TCF-4.

Application

Immunocytochemistry/Immunohistochemistry:
A previous lot of this antibody is reported to show positive immunostaining for TCF-4 in cells fixed with 4% paraformaldehyde/PBS (Amir, A. L., 2003).

Immunoprecipitation:
A previous lot of this antibody was reported to immunoprecipitate TCF-4 from SW620 whole cell lysate as a complex with β-Catenin (Persad, S., 2001).

Supershift Assay:
A previous lot of this antibody was reported to supershift TCF-4:DNA complexes in electrophoretic mobility shift assays (EMSA) (Amir, A. L., 2003).

Target description

Mr 66 kDa

Physical form

Format: Purified

Purified mouse monoclonal IgG2a in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Quality

Routinely evaluated by western blot on RIPA lysates of Cos-1 cells expressing transfected TCF-4.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected TCF-4 in RIPA lysates of Cos-1 cells expressing transfected TCF-4 (Catalog # 21-174).

Linkage

Replaces: 04-1080

Analysis Note

Control
DLD1 (human colorectal adenocarcinoma), Cos-1 cells or HCT116 (human colorectal carcinoma) cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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