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  • 05-623 - Anti-RNA polymerase II Antibody, clone CTD4H8

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05-623 Sigma-Aldrich

Anti-RNA polymerase II Antibody, clone CTD4H8

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RH-RN
clone   CTD4H8, monoclonal
biological source   mouse
application(s)   ChIP: suitable (ChIP-seq)
  western blot: suitable
species reactivity   mouse, Saccharomyces cerevisiae, rat, human
shipped in   dry ice
isotype   IgG1
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NP_000928
UniProt accession no.   P24928

Description

General description

RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

Specificity

This antibody recognizes Phospho- and non-phospho-RNA polymerase II.

Immunogen

Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Target description

210-220 kDa

Physical form

Format: Purified

Protein G Chromatography

Purified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by western blot on HeLa nuclear extracts.

Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts

Analysis Note

Control
A431 nuclear extract.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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