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  • 05-636 - Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

05-636 Sigma-Aldrich

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.

Synonym: H2AXS139P, Histone H2A.X (phospho S139)

  •  eCl@ss 32160702



Related Categories Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, Miscellaneous, Neuroscience,
packaging   200 µg (05-636-AF555)
NCBI accession no.   NP_002096
UniProt accession no.   P16104
biological source   mouse
mfr. no.   Upstate
application(s)   ChIP: suitable
  immunocytochemistry: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  western blot: suitable
isotype   IgG1
clone   JBW301, monoclonal
shipped in   ambient



Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

17 kDa

Analysis Note

UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue


Additional Referenced Applications:
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.

Research Category
Epigenetics & Nuclear Function

Research Sub Category

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.


peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X


Replaces: MABE205

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.


Immunoblot Analysis: 0.05-1 μg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.


Recognizes Histone H2A.X phosphorylated at Ser139.

Physical form

Format: Purified

Immunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.

Protein G Purified

Storage and Stability

Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Peer-Reviewed Papers


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