• USA Home
  • 05-717 - Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2

EMAIL THIS PAGE TO A FRIEND
05-717 Sigma-Aldrich

Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2

Anti-Adenosine Receptor A2a Antibody, clone 7F6-G5-A2 is an antibody against Adenosine Receptor A2a clone 7F6-G5-A2 for use in FC, IH & WB.

Synonym: adenosine A2 receptor, adenosine A2a receptor, adenosine receptor subtype A2a

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   200 µg (05-717)
application(s)   flow cytometry: suitable
  immunohistochemistry: suitable
  western blot: suitable
biological source   mouse
clone   7F6-G5-A2, monoclonal
  monoclonal
isotype   IgG2a
mfr. no.   Upstate
shipped in   wet ice
species reactivity   human, rabbit, mouse, canine, rat
gene symbol   ADORA2A(135)
Species Reactivity Notes   Human, mouse, rat, rabbit, and dog. Cross-reactivity with guinea pig also reported.
gene symbol   ADORA2
  RDC8
  hA2aR

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~43 kDa

Analysis Note

Control
Brain tissue

Application

Immunohistochemistry: 1 μg/mL of a previous lot detected Adenosine Receptor A2A in paraformaldehyde-fixed mouse brain striatal sections. Not detected in Adenosine Receptor A2A knockout mice.

FACS Analysis: A previous lot of this antibody has been reported by an independent laboratory to detect Adenosine Receptor A2A in transfected HEK293 cells (Koshiba, M., 1999).

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Neurodegenerative Diseases

General description

Adenosine receptors, a class of purinergic G-protein coupled receptors with adenosine as endogenous ligands mediate several receptor subtype-specific physiological processes that include cardiac rate, smooth muscle tone, platelet aggregation, inflammation, cell growth and death, and neurotransmission. The activity of A2A adenosine receptor is mediated by G proteins which activate adenylyl cyclase. It is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine. Adenosine receptor A2A-R contributes to motoneuron survival by transactivating the tyrosine kinase receptor TrkB and may suggest a new strategy for using this mechanism in neurodegenerative diseases.

Immunogen

6His-tagged full-length human recombinant A2A receptor. Epitope mapped to SQPLPGER in the third intracellular loop. Clone 7F6-G5-A2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Routinely evaluated by immunohistochemistry.

Western Blot Analysis:
A 1:1000–1:5000 dilution of this lot of antibody detected Adenosine Receptor A2A in HEK293 cells expressing canine A1/A2A receptor chimeras (Rosin, D.L., 1998).

Specificity

This antibody recognizes adenosine receptor A2A, Mr 43 kDa.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2ak in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Making Headway
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?