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05-729 Sigma-Aldrich

Anti-Bcl2 Antibody, clone 100

Detect Bcl2 using this Anti-Bcl2 Antibody, clone 100 validated for use in FC, IH & WB.

Synonym: B-cell CLL/lymphoma 2, B-cell lymphoma protein 2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, B-BI, Primary Antibodies
clone   100, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, human, feline, canine
shipped in   dry ice
isotype   IgG1
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_000633
UniProt accession no.   P10415
Gene Information   human ... BCL2(596)

Description

General description

The Bcl name is derived from B-cell chronic lymphocytic leukemia, and was given to genes involved in that disease. Members of the Bcl2 family share homologous domains (BH) through which they are able to homo- and hetero-dimerize. Apoptosis/survival is regulated by the relative balance of pro-apoptotic (Bax, BclXS, Bak, Bad) and anti-apoptotic (Bcl2, BclXL, Mcl-1, Dad-1) family members, and their heterodimerization. Bcl2 plays an important role in cytochrome c release from the mitochondria; it also binds Apaf1, inhibiting Apaf-1 association with caspase-9. Bcl-2 overexpression in indicated in a number of human cancers, including melanoma, breast, prostate, and lung, and it correlates with resistance to cancer treatment (presumably due to decreased apoptosis). Bcl-2 has also been implicated in schizophrenia and autoimmune disorders.

Specificity

Predicted to cross-react with feline and canine, based on sequence homology.

This antibody recognizes Bcl2, Mr 26 kDa.

Immunogen

Epitope: a.a. 41-54

Synthetic peptide corresponding to amino acids 41-54 of human Bcl2 protein. The immunizing sequence is identical in canine and feline.

Application

Immunohistochemistry:
An independent laboratory has reported that this antibody detects Bcl2 in paraffin and acetone-fixed tissues.

FACS Analysis:
Recommended

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

Target description

26 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 70% storage buffer PBS with 0.1% sodium azide and 30% glycerol. Store at -20ºC.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by western blot on Raji RIPA cell lysate.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected Bcl2 in a Raji RIPA cell lysate.

Linkage

Replaces: 04-436

Analysis Note

Control
Tonsil tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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