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06-1038 Sigma-Aldrich

Anti-Unc84B/SUN2 Antibody

clone, 0.5 mg/mL, from rabbit

Synonym: Rab5-interacting protein, Sad1 unc-84 domain protein 2, Sad1/unc-84 protein-like 2, nuclear envelope protein, unc-84 homolog B, unc-84 homolog B (C. elegans)

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, canine, human, horse
species reactivity (predicted by homology)   mouse (87%), horse, rat (87%), dog
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
concentration   0.5 mg/mL
NCBI accession no.   NP_056189
UniProt accession no.   Q9UH99
Gene Information   human ... SUN2(25777)

Description

General description

UNC84B (Protein UNC-84 homolog B or SUN2), is a single-pass nuclear envelope transmembrane protein responsible for nuclear migrations that are essential for development. UNC-84B has a predicted transmembrane domain and a C-terminal region with similarity to the S. pombe spindle pole body protein Sad1. UNC-84B interacts with RAB5A and is widely expressed, and highly expressed in the heart, lungs and muscles. UNC-84 may function to facilitate a nuclear-centrosomal interaction required for nuclear migration and anchorage.

Specificity

This antibody recognizes Unc84B/SUN2 at the N-terminus.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to human Unc84B/SUN2 at and around the N-terminus.

Application

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated Unc84B/SUN2 from 500 µg of U2OS cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

This Anti-Unc84B/SUN2 Antibody is validated for use in Immunoprecipitation and Western Blotting for the detection of Unc84B/SUN2.

Target description

~80 kDa

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blot (SNAP ID) Analysis: 1:250 dilution of this antibody detected Unc84B/SUN2 on 10 µg of U2OS cell lysate.

Analysis Note

Control
U2OS cell lysate.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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