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06-248 Sigma-Aldrich

Anti-IRS1 Antibody

clone, Upstate®, from rabbit

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IQ-IZ, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, human, rat
shipped in   dry ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Upstate®
NCBI accession no.   NP_005535
UniProt accession no.   P35568
Gene Information   human ... IRS1(3667)

Description

General description

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

Specificity

Recognizes IRS1, Mr 160-185kDa..

Immunogen

Carboxy-terminal 14 amino acid peptide ([C]YASINFQKQPEDRQ) of rat liver IRS1

Application

Anti-IRS1 Antibody is an antibody against IRS1 for use in IP & WB.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Target description

165 kDa

Physical form

ImmunoAffinity Purified

Purified Rabbit IgG in buffer containing 0.2 M Trisglycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, 5 mg/mL BSA, and 0.05% sodium azide.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on NIH3T3 cell lysate.

Analysis Note

Control
Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 Cell Lysate. Use 20 µg per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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