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06-284 Sigma-Aldrich

Anti-Nitrotyrosine Antibody

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity (predicted by homology)   all
shipped in   dry ice
isotype   IgG
antibody product type   primary antibodies
mfr. no.   Upstate®

Description

General description

Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others.

Specificity

Recognizes proteins which are nitrated.

Immunogen

Nitrated KLH

Application

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases

Target description

Predicted MW for nitrated-BSA: 85 kDa

Physical form

Format: Purified

ImmunoAffinity Purified

Purified Rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, 0.05% sodium azide, and 10 mg/mL BSA

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

routinely evaluated by immunoblot on nitrotyrosine immunoblotting controls (Catalog #12-354)

Analysis Note

Control
Immunohistochemistry: Human Alzheimer disease brain sections; mouse liver
Immunoblotting: Nitrated-BSA
Indirect ELISA: Nitrated-BSA
Indirect immunofluorescence: A549 cells metabolically labeled with 3-nitrotyrosine

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Acute and Chronic Inflammation: Microglia in Neuroprotection and Neurodegeneration

The term neurodegeneration characterizes a chronic loss of neuronal structure and function leading to progressive mental impairments. The inci­dence of neurodegeneration generally increases with age....
Chandra Mohan
Ph.D. MilliporeSigma, Temecula, CA
Keywords: AGE, Angiogenesis, Anti-inflammatory agents, Apoptosis, Central Nervous System, Degradations, Diseases, Gene expression, Growth factors, Inflammation, Neurodegenerative Diseases, Neurotoxins, Neurotransmission

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

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