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06-413 Sigma-Aldrich

Anti-NFκB p52 Antibody

Detect NFκB p52 with Anti-NFκB p52 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in IP & WB.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_019408
UniProt accession no.   Q00653

Description

General description

The NFκB transcription factor was originally identified as a protein complex consisting of a 65 kDa DNA binding subunit and an associated 50 kDa protein. The 65 kDa subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a 100 kDa protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Specificity

Recognizes NFκB p52 and its precursor.

Immunogen

KLH conjugated synthetic peptide (CYNPGLDGIIEYDDFK) contained within amino acids 4-19 of human NF κB, p52

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Target description

52 kDa, and precursor (100 kDa)

Physical form

Format: Purified

Protein A Purified immunoglobulin in in 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% NaN3 as a preservative.

Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

routinely evaluated by immunoblot on RIPA lysates from Raji cells

Analysis Note

Control
Raji cell lysate, Jurkat whole cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Acute and Chronic Inflammation: Microglia in Neuroprotection and Neurodegeneration

The term neurodegeneration characterizes a chronic loss of neuronal structure and function leading to progressive mental impairments. The inci­dence of neurodegeneration generally increases with age....
Chandra Mohan
Ph.D. MilliporeSigma, Temecula, CA
Keywords: AGE, Angiogenesis, Anti-inflammatory agents, Apoptosis, Central Nervous System, Degradations, Diseases, Gene expression, Growth factors, Inflammation, Neurodegenerative Diseases, Neurotoxins, Neurotransmission

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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