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06-429-I Sigma-Aldrich

Anti-IGF-IRα subunit Antibody

from chicken, purified by affinity chromatography

Synonym: Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, IGF-I receptor, CD221, Insulin-like growth factor 1 receptor alpha chain, Insulin-like growth factor 1 receptor beta chain, IGF-IRα subunit

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, I-IG, Primary Antibodies
clone   polyclonal
biological source   chicken
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, human, human
species reactivity (predicted by homology)   rhesus macaque (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
shipped in   wet ice
isotype   IgY
Quality Level   100
antibody product type   primary antibodies
purified by   affinity chromatography
NCBI accession no.   NP_000866
UniProt accession no.   P08069
Gene Information   human ... IGF1R(3480)

Description

General description

Insulin-like growth factor 1 receptor (EC:2.7.10.1; UniProt P08069; also known as CD221, IGF-I receptor, Insulin-like growth factor I receptor) is encoded by the IGF1R (also known as CD221, IGFIR, IGFR, JTK13) gene (Gene ID 3480) in human. The insulin-like growth factor 1 (IGF-1) receptor has tyrosine kinase that is activated by IGF-1 and IGF-2 binding. IGF-1 receptor is initially produced as a precursor protein with an aignal peptide sequence (aa 1-30). Post-translational cleavages produce one extracellular alpha (aa 31-736) chain and one transmembrane beta chain (aa 741 – 1367) linked together by a disulfide bond. Two sets of alpha-beta dimers then form the functional tetrameric receptor complex via a disulfide linkage between their alpha chain. Upon ligand binding, the alpha chains induce the tyrosine auto-phosphorylation of the beta chains, which in turn triggers cell type-specific downstream signaling cascade of events.

Immunogen

Epitope: Third Fibronectin Type III (FnIII 3) domain

KLH-conjugated synthetic peptide [C-KYADGTIDIEEVTENPKT] corresponding to amino acid residues 672-689 of human IGF-IR alpha chain.

Application

Immunoprecipitation Analysis: 5.0 µg from a representative lot immunoprecipitated human IGF-IRα subunit in 0.5 mg of human IGF-IR-expressing C4 cell lysate.
Immunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected IGF-IRα subunit in human IGF-IR-expressing C4 cells.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-IGF-IRα subunit Antibody is validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry for the detection of IGF-IRα subunit.

Target description

~110/180 kDa observed

Physical form

Affinity Purfied

Purified chicken polyclonal IgY antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected IGF-IRα subunit in 10 µg of human IGF-IR-expressing murine fibroblast LISN C4 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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