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06-536 Sigma-Aldrich

Anti-Bak Antibody, NT

clone, Upstate®, from rabbit

Synonym: Apoptosis regulator BAK, BCL2-antagonist/killer 1, BCL2-like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like 7 protein, pro-apoptotic protein BAK

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, B-BI, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, human, rat
shipped in   dry ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_001188
UniProt accession no.   Q16611
Gene Information   human ... BAK1(578)

Description

General description

Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.

Specificity

Specific for Bak, MW ~28 kDa.

Immunogen

Epitope: N-terminus

Synthetic peptide (SEEQVAQQDTEEVFRSC) corresponding to amino acid residues 23-38 of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH.

Application

Detect Bak using this Anti-Bak Antibody, NT validated for use in IC, IH, IP & WB.

Immunohistochemistry (Paraffin):
5 μg/mL of a previous lot detected Bak in paraffin-embedded rat kidney tissue.

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated Bak from 500 μg mouse 3T3 cell lysate.

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

Target description

28 kDa

Physical form

Format: Purified

Protein A Purfied

Purified rabbit polyclonal in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen liquid.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot on HEK293 lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Bak on 10 µg of HEK293 lysates.

Linkage

Replaces: 04-433

Analysis Note

Control
Included Positive Antigen Control: Catalog # 12-301, non-stimulated human A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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