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06-549 Sigma-Aldrich

Anti-Myc Tag Antibody

Synonym: Transcription factor p64, avian myelocytomatosis viral oncogene homolog, myc proto-oncogene protein, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)

  •  eCl@ss 32160702

  •  NACRES NA.43

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Properties

Related Categories Alphabetical Index, Antibodies, MUE-MZ, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   human, human
shipped in   dry ice
isotype   IgG
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_002467.3
UniProt accession no.   P01106

Description

General description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein′s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Specificity

Other species cross-reactivity not tested.

Recognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.

Immunogen

Epitope: a.a. 409-420

Synthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).

Application

Immunocytochemistry:
This antibody is reported to detect Myc-tagged proteins in transfected cells.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Target description

Predicted molecular weight for human Myc: 49 kDa Obeserved molecular weight may vary.

Physical form

Format: Purified

Protein A chromatography

Purified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blotting:
1:500 dilution of this antibody detected myc-PP2A on 10 μg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

Analysis Note

Control
Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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