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06-570 Sigma-Aldrich

Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker

clone, Upstate®, from rabbit

Synonym: H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, HF-HI, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, mouse
shipped in   wet ice
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NP_003484.1
UniProt accession no.   Q16695
Gene Information   human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

This antibody recognizes Histone H3 when phosphorylated at Ser10.

Immunogen

Epitope: Ser10

Linear peptide corresponding to human Histone H3 at Ser10.

Application

Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker is a Rabbit Polyclonal Antibody for detection of Histone H3 phosphorylated at serine 10.This highly published Ab, also known as Anti-H3S10p, has been validated in ICC, IP & WB.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.
Beadlyte Assay Analysis: A representative lot recognizes Histone H3 phosphorylated on Ser10 by Luminex assay.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Target description

17 kDa

Physical form

Format: Purified

Protein A Purfied

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.

Storage and Stability

2-8°C for up to 6 months.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in Colcemid treated HeLa acid extract.
Western Blot Analysis: 0.5 – 1 µg/ml of this antibody detected Histone H3 on 10 µg of Colcemid treated HeLa acid extract.

Analysis Note

Control
Colcemid treated HeLa acid extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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