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06-570 Millipore

Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker

Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker is a Rabbit Polyclonal Antibody for detection of Histone H3 phosphorylated at serine 10.This highly published Ab, also known as Anti-H3S10p, has been validated in ICC, IP & WB.

Synonym: H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   200 µg (06-570-AF555)
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
biological source   rabbit
clone   polyclonal
mfr. no.   Upstate
shipped in   wet ice
species reactivity   human, mouse
gene symbol   HIST1H3A(8350)
  HIST1H3B(8358)
  HIST1H3C(8352)
  HIST1H3D(8351)
  HIST1H3E(8353)
  HIST1H3F(8968)
  HIST1H3G(8355)
  HIST1H3H(8357)
  HIST1H3I(8354)
  HIST1H3J(8356)
  HIST3H3(8290)
  H3FA
  H3FB
  H3FC
  H3FD
  H3FF
  H3FH
  H3FI
  H3FJ
  H3FK
  H3FL
  H3FT
  H3T
  MGC126886

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

17 kDa

Analysis Note

Control
Colcemid treated HeLa acid extract

Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.
Beadlyte Assay Analysis: A representative lot recognizes Histone H3 phosphorylated on Ser10 by Luminex assay.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Epitope: Ser10

Linear peptide corresponding to human Histone H3 at Ser10.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in Colcemid treated HeLa acid extract.
Western Blot Analysis: 0.5 – 1 µg/ml of this antibody detected Histone H3 on 10 µg of Colcemid treated HeLa acid extract.

Specificity

This antibody recognizes Histone H3 when phosphorylated at Ser10.

Physical form

Format: Purified

Protein A Purfied

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.

Storage and Stability

2-8°C for up to 6 months.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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