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06-659 Sigma-Aldrich

Anti-phospho-c-Jun (Ser73) Antibody

Anti-phospho-c-Jun (Ser73) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-c-Jun (Ser73) also known as Activator protein 1 & has been validated in ICC, IP & WB.

Synonym: Activator protein 1, Jun activation domain binding protein, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, enhancer-binding protein AP1, jun oncogene, v-jun sarcoma virus 17 oncogene homolog, v-jun sarcoma virus 17 oncogene homolog

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (06-659)
gene symbol   JUN(3725)
trade name   Upstate
brand family   Upstate
format   Affinity Purified
molecular weight   ~40 kDa
application(s)   Immunocytochemistry
  Immunoprecipitation
  Western Blotting
clone   Polyclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Rabbit
species reactivity   Human
  Mouse
Species Reactivity Notes   Human, Mouse. Predicted to react with Rat, Bovine, Chicken, Zebrafish, Xenopus based on 100% sequence homology.
NCBI accession no.   NP_002219.1
UniProt accession no.   P05412
gene symbol   AP1
  c-Jun
  p39
shipped in   1
Quality Assurance   Evaluated by Western Blotting in Anisomycin treated NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Phospho-C-Jun (Ser73) in 10 µg of Anisomycin treated NIH/3T3 cell lysate.

Description

Analysis Note

Control
Untreated and anisomycin-treated NIH/3T3 cell lysate.

Application

Western Blot Analysis :
A previous lot of this antibody detected 10 ng of phosphorylated gst-Jun, but did not detect 10 ng of nonphosphorylated Jun, as reported by an independent laboratory.

General description

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced

signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

Immunogen

KLH-conjugated linear peptide corresponding to the human Phospho-C-Jun (Ser73) near the N-terminus.

Specificity

This antibody detects phospho-c-Jun. May also detect phospho-JunD in properly treated cells since the sequence homology is identical (13/13).

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Peer-Reviewed Papers
15

References

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