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06-866 Sigma-Aldrich

Anti-acetyl-Histone H4 Antibody

Synonym: H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ChIP: suitable
  immunocytochemistry: suitable
  western blot: suitable
species reactivity   eukaryotes, Tetrahymena sp., human, human, Tetrahymena sp.
shipped in   dry ice
antibody form   serum
isotype   IgG
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Upstate®
NCBI accession no.   NM_175054
UniProt accession no.   P62805

Description

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Specificity

Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.

Other species not tested, but expected to cross-react since Histone H4 is well conserved.

Immunogen

Epitope: a.a. 2-19

KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.

Application

Chromatin Immunoprecipitation:
5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Target description

10 kDa

Physical form

Unpurified

Whole antiserum containing 0.05% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.

Western Blot Analysis:
1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4.

Analysis Note

Control
TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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