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06-911 Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys14) Antibody

Use Anti-acetyl-Histone H3 (Lys14) Antibody (Rabbit Polyclonal Antibody) validated in ICC, IP, WB to detect acetyl-Histone H3 (Lys14) also known as H3K14Ac, Histone H3 (acetyl K14).

Synonym: H3K14Ac, Histone H3 (acetyl K14), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, human, eukaryotes
shipped in   dry ice
isotype   IgG
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_003493
UniProt accession no.   Q16695

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Recognizes Histone-H3, Mr 17 kDa, acetylated at position 14.

This peptide sequence is identical in a wide range of animal and plant species.

Immunogen

KLH-conjugated synthetic peptide (ARKSTGG[AcK]APR-GC) corresponding to amino acids 7-17 of Histone-H3.

Application

Immunoprecipitation:
1 μg of a previous lot immunoprecipitated acetylated Histone-H3 from 500 μg of an acid precipitate of sodium butyrate treated HeLa cells.

Immunocytochemistry:
Not determined.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Target description

Approx. 17 kDa

Physical form

Format: Purified

Protein A Purfied

Purified rabbit polyclonal IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide and 30% glycerol. Store at -20°C.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on sodium butyrate treated acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.5-1.0 μg/mL of this lot detected acetylated Histone-H3 in acid extracted proteins from HeLa cells. Sodium butyrate, an inhibitor of histone deacetylases, was used to enhance detection of acetylated Histone-H3.

Analysis Note

Control
Sodium butyrate treated acid extracted proteins from HeLa cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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