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  • 06-942 - Anti-acetyl-Histone H3 (Lys9) Antibody

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06-942 Sigma-Aldrich

Anti-acetyl-Histone H3 (Lys9) Antibody

Synonym: H3K9Ac, Histone H3 (acetyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AC-AC, Alphabetical Index, Antibodies, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ChIP: suitable (ChIP-seq)
  ChIP: suitable
  dot blot: suitable
  flow cytometry: suitable
  western blot: suitable
species reactivity   rat, mouse, human, mouse, rat, human
shipped in   dry ice
isotype   IgG
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Upstate®
NCBI accession no.   NP_003520
UniProt accession no.   Q16695
Gene Information   human ... HIST1H3F(8968)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

Histone H3 acetylated at position 9.

although this peptide sequence is identical in a wide range of animal and plant species.

Immunogen

Epitope: Surrounding H3K9ac

KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9.

Application

Chromatin Immunoprecipitation/ChIP-seq Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys9) in 5 µg of HeLa cell lysate.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl-Histone H3 (Lys9) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected acetyl-Histone H3 (Lys9) in 1X10E6 HEK293 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Target description

~17 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein A Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected acetyl-Histone H3 (Lys9) in 10 µg of sodium butyrate treated HeLa cell lysate.

Linkage

Replaces: 04-1003

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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