• USA Home
  • 07-131-AF488 - Anti-Sirt1 (Sir2) Antibody, Alexa Fluor 488 Conjugate

EMAIL THIS PAGE TO A FRIEND
07-131-AF488 Sigma-Aldrich

Anti-Sirt1 (Sir2) Antibody, Alexa Fluor 488 Conjugate

Use Anti-Sirt1(Sir2) Antibody (Rabbit Polyclonal Antibody) is published (more than 30 citations) and validated in ICC, IP, WB to detect Sirt1(Sir2) also known as Silencing information regulator 2-like, sirtuin 2.

Synonym: NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2alpha, Sir2, mSIR2a, SirtT1 75 kDa fragment, 75SirT1, Sirt1(Sir2), Alexa Fluor 488 Conjugate

  •  eCl@ss 32160702

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
conjugate   ALEXA FLUOR® 488
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
species reactivity   human, mouse
shipped in   wet ice
antibody product type   primary antibodies
NCBI accession no.   NP_062786
UniProt accession no.   Q923E4

Description

General description

NAD-dependent protein deacetylase sirtuin-1 (UniProt Q923E4; also known as Sir2, mSIRT2a, NAD-dependent deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, Sir2-like 1, SIR2-like protein 1, SIR2alpha) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID 93759) in murine species. Sirtuins constitute a family of NAD+-dependent deacetylases found in nearly all organisms. Sirtuins have been implicated in the regulation of aging, transcription, apoptosis, and stress resistance. Full-length SirT1 (FLSirT1) plays an important role in human cartilage homeostasis and chondrocyte survival by mediating the expression of major cartilage anabolic components, collagen 2(I) & aggrecan. The generation of the transcriptionally inactive 75-kDa fragment (75SirT1) via site-specific cleavage of FLSirT1 at amino acid 533 by cathepsin B is an anti-apoptotic mechanism to promote human osteoarthritic (OA) chondrocytes survival following exposure to proinflammatory cytokines. The 5SirT1 fragment is shown to associate with cytochrome c and likely blocks downstream apoptosome assembly. Phosphorylation plays an important role in modulating SirT1 activity and cellular functions. Multiple kinases and their corresponding SirT1 phosphorylation sites have been identified, including pT344 targeted by AMPK, pT530 and pS540 targeted by Cdk1/Cyclin B, pS46 (mouse) or pS47 (human) targeted by JNK1, pT522 targeted by DYRK1A & DYRK3, as well as pS27 & pS47 targeted by CaMKKβ.

Specificity

This antibody recognizes Sirt1.

Immunogen

Epitope: a.a. 1-131

GST-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Sirt1(Sir2) in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 07-131) is shown to be suitable also for Western blotting, immunoprecipitation, and ChIP applications.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Sirt1 (Sir2) Antibody, Alexa Fluor® 488 Conjugate is validated for use in Immunocytochemistry for the detection of Sirt1 (Sir2).

Target description

~110 kDa observed

Physical form

Affinity Purfied

Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Sirt1(Sir2) in A431 cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?