• USA Home
  • 07-145 - Anti-phospho-Histone H3 (Ser28) Antibody

EMAIL THIS PAGE TO A FRIEND
07-145 Sigma-Aldrich

Anti-phospho-Histone H3 (Ser28) Antibody

Synonym: Histone H3.1t, H3/t, H3t, H3/g

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, HF-HI, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ChIP: suitable
  dot blot: suitable
  immunocytochemistry: suitable
  inhibition assay: suitable (peptide)
  western blot: suitable
species reactivity   human, mouse, human, mouse
shipped in   wet ice
antibody product type   primary antibodies
Quality Level   100
purified by   affinity chromatography
NCBI accession no.   NP_003484.1
UniProt accession no.   Q16695

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

This antibody is expected to demonstrate broad species reactivity due to sequence conservation.

This antibody recognizes Histone H3 phosphorylated at Ser28.

Immunogen

Epitope: Histone H3 phosphorylated at Ser28

KLH-conjugated linear peptide corresponding to Histone H3 phosphorylated at Ser28.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: A 1:2,000 dilution from a representative lot detected phospho-Histone H3 (Ser28) in 10 µg of untreated and colcemid treated HeLa acid extract.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho-Histone H3 (Ser28) in HeLa cells.

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-Histone H3 (Ser28) in colcecmid treated HeLa cell lysate.

Dot Blot Specificity Analysis: A representative lot of this antibody detected peptides corresponding to phospho-Histone H3 (Ser28), but not peptides corresponding to other unmodified and modifed Histones.

Peptide Inhibition Analysis: A 1:500 dilution form a representative was blocked by phospho-Histone H3 (Ser28) peptides, but not non-specific peptides.

Target description

~17 kDa observed

Physical form

Affinity Purfied

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in HeLa acid extract.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected phospho-Histone H3 (Ser28) in 10 µg of HeLa acid extract.

Analysis Note

Control
HeLa acid extract

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?