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07-1464 Sigma-Aldrich

Anti-Rac1 Antibody

clone, from rabbit

Synonym: Ras-related C3 Botulinum Toxin Substrate 1, p21-Rac1, Ras-like Protein TC25

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, RAB-RAD
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   rat, human, mouse
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NM_006908.4
UniProt accession no.   P63000
Gene Information   human ... RAC1(5879)

Description

General description

Rac is a member of the Rho family of small GTP-binding proteins, and plays roles in cytoskeletal actin organization as well as transformation. Rac is activated downstream of cdc42, or independently by Ras signaling, and in turn Rac stimulates Rho. Activation of Rac results in the formation of lamellipodia, whereas cdc42 stimulates filopodia formation, and Rho stimulates formation of actin stress fibers. Effectors of the Rac pathway include NADPH Oxidase and the SAPK1/JNK family of kinases. The latter pathway is activated by the PAK kinases, which are direct targets of Rac.

Specificity

Recognizes Rac1, does not cross react with Cdc42. Rac1 is a member of the Rho family of small GTPases. Small GTPases act as molecular switches in regulating a variety of biological response pathways by cycling from the GDP-bound (inactive) and GTP-bound (active) states. Rac regulates actin polymerization to form lamellipodia.

Immunogen

Synthetic peptide corresponding to amino acids 130-141 of human Rac1 (KEKKLTPITYPQ-C).

Application

Anti-Rac1 Antibody is an antibody against Rac1 for use in WB.

Optimal working dilutions must be determined by end user.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Target description

21 kDa

Physical form

Format: Purified

Protein A Purfied

Purified rabbit polyclonal IgG in 0.02 M PBS with 0.15 M NaCl, pH 7.6 and 0.1% sodium azide as a preservative

Storage and Stability

Stable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected RAC1 on 10 μg of L6 lysates.

Linkage

Replaces: AB4202

Analysis Note

Control
Immunoblot/Immunoprecipitation: L6 Cell Lysate or Rat brain microsomal protein preparation
Immunohistochemistry: Rat brain sections

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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