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  • 07-158 - Anti-KDR/Flk-1/VEGFR2 Antibody

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07-158 Sigma-Aldrich

Anti-KDR/Flk-1/VEGFR2 Antibody

Synonym: Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, FLK-1, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, CD309

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, K-KH, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   mouse, mouse
species reactivity (predicted by homology)   rat (based on 99% sequence homology), human (based on 92% sequence homology)
shipped in   wet ice
antibody product type   primary antibodies
purified by   affinity chromatography
mfr. no.   Upstate®
NCBI accession no.   NP_002244
UniProt accession no.   P35968
Gene Information   human ... KDR(3791)

Description

General description

The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.

Specificity

This antibody recognizes KDR/Flk-1/VEGFR2 at the cytoplasmic domain.

Immunogen

GST-tagged recombinant protein corresponding to mouse KDR/Flk-1/VEGFR2 at the cytoplasmic domain.

Application

Immunohistochemistry Analysis: 1:100 dilution from a representative lot detected KDR/Flk-1/VEGFR2 in mouse embryo vascular endothelium tissue.

Target description

~150/180 kDa observed. 180 kDa is the mature form and 150 kDa is the immature unmodified form. In human and mouse cells the mature form of VEGFR2 is ~220-225 kDa Faint band at ~225 kDa may be endogenous VEGFR2 in PAE cells.

Quality

Evaluated by Western Blot in PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.

Western Blot Analysis: 1:1,000 dilution of this antibody detected KDR/Flk-1/VEGFR2 on 10 µg of PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF.

Analysis Note

Control
PAE cells transfected with CSF/VEGFR2 chimera and treated with CSF

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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