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07-164 Sigma-Aldrich

Anti-phospho-H2A.X (Ser139) Antibody

Anti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.

Synonym: H2A histone family, member Q, H2ac, H2ac, histone 2, histone IIa, histone cluster 2

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Properties

Related Categories Antibodies, Primary Antibodies More...
trade name   Upstate
brand family   Upstate
format   Purified
control   Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.
molecular weight   15 kDa
application(s)   Immunoprecipitation
  Western Blotting
  ChIP-seq
isotype   IgG
clone   Polyclonal Antibody
purification method   Protein A chromatography
epitope   a.a. 134-142
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Rabbit
species reactivity   Vertebrates, Human
NCBI accession no.   NP_002096
UniProt accession no.   P16104
gene symbol   HIST2H2AC(8338)
  H2A-GL101
  H2A/Q
  H2A/R
  H2A
  H2AFQ
  MGC74460
modifications   Phosphorylation
material size   200 µg
shipped in   wet ice
storage conditions   Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Description

Background information

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.

Physical form

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Application

Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.

Specificity

Histone H2A.X phosphorylated at Ser139.

Immunogen

KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.

Species Reactivity Notes

Human; broad species cross-reactivity expected based on conservation of sequence homology.

Quality Assurance

Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.

Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Peer-Reviewed Papers
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References

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