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07-209-I Sigma-Aldrich

Anti-phospho-SHC Antibody (Tyr239, Tyr240)

Detect SHC tyr239 and tyr240 phosphorylation using this rabbit polyclonal Anti-phospho-SHC (Tyr239, Tyr240) antiboody, Cat. No. 07-209-I, validated for use in Western Blotting.

Synonym: SH2 domain protein C1, tyrosine-phosphorylated, SHC-transforming protein 1, tyrosine-phosphorylated, SHC-transforming protein 3, tyrosine-phosphorylated, SHC-transforming protein A, tyrosine-phosphorylated, Src homology 2 domain-containing-transforming protein C1, tyrosine-phosphorylated, p46Shc (pTyr194/pTyr195), p52Shc (pTyr239/pTyr240), p66Shc (pTyr349/pTyr350)

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Affinity Purified
molecular weight   ~52 kDa observed. 62.82/51.61/46.67/40.42/62.89/51.68 kDa (human isoform p66Shc/p52Shc/p46Shc/5/6/7), 62.61/51.41/46.47 kDa (mouse isoform p66Shc/p52Shc/p47Shc), and 51.50 kDa (rat) calculated.
application(s)   Western Blotting
clone   Polyclonal Antibody
purification method   Affinity purified.
concentration   Please refer to lot specific datasheet.
host   Rabbit
species reactivity   Human
NCBI accession no.   NP_892113
UniProt accession no.   P29353
gene symbol   SHC1(6464)
  SHC
  SHCA
modifications   Phosphorylation
  Phosphorylation
material size   100 μg
shipped in   ambient
storage conditions   Stable for 1 year at 2-8°C from date of receipt.

Description

Background information

SHC-transforming protein 1 (UniProt P29353; also known as SH2 domain protein C1, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1) is encoded by the SHC1 (also known as SHC, SHCA) gene (Gene ID 6464) in human. Originally identified as an SH2-containing proto-oncogene involved in growth factor signaling, Shc proteins function as adaptor molecules recruited by numerous receptors (e.g. integrins, GPCRs, and receptors for growth factors, antigens, cytokines, and hormones) for signling. In addition to the ubiquitously expressed Shc (a.k.a. ShcA by SHC1/SHCA gene), there exist also ShcB (by SHC2/SHCB gene) and ShcC (by SHC3/SHCC gene), which are predominantly expressed in neuronal cells. Alternative splicings result in six ShcA isoforms, including p66Shc, p52Shc, and p46Shc. All ShcA isoforms possess two distinct domains, PTB (a.a. 156-339) and SH2 (a.a. 488-579), that bind phosphotyrosine-containing sequences, as well as a central region (CH1; a.a. 340-487) that contains critical tyrosine phosphorylation sites. The phosphotyrosine-binding (PTB) domain is also referred to as phosphotyrosine-interaction domain or PID.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Specificity

Numbering of the target pTyr residues varies depending on the species and spliced isoforms. Target pTyr residues this rabbit polyclonal detects correspond to pTyr349/pTyr350 of human spliced isoform 6 and p66Shc, pTyr239/pTyr240 of human spliced isoform 7 and p52Shc, pTyr194/pTyr195 of isoform p46Shc, and pTyr135/pTyr136 of isoform 5 reported by UniProt (P29353).

Immunogen

KLH-conjugated linear peptide corresponding to a target site sequence surrounding phosphorylated Tyr239 and Tyr240 of human spliced isoform p52Shc.

Species Reactivity Notes

Human. Predicted to react with Bovine, Chicken, Mouse, Rat, Rhesus Macaque based on 100% sequence homology.

Quality Assurance

Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected SHC tyrosine phosphorylation induction in 10 µg of lysate from EGF-stimulated HEK293 cells.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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