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07-315 Sigma-Aldrich

Anti-SGK1 Antibody

This Anti-SGK1 Antibody is validated for use in IP, WB for the detection of SGK1.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, rat
shipped in   dry ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_005627
UniProt accession no.   O00141
Gene Information   human ... SGK1(6446)

Description

Specificity

Recognizes SGK1.

Immunogen

peptide corresponding to amino acids 399-412 (C-GKSPDSVLVTASVK) of human SGK1

Application

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Target description

~50 kDa

Physical form

Format: Purified

Immunoaffinity Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide and 1.75mg/ml BSA before the addition of glycerol to 30%

Protein A Purfied

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

routinely evaluated by immunoblot on RIPA lysates from A431 and Rat 2 cells

Linkage

Replaces: 04-1027

Analysis Note

Control
Rat 2 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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