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  • 07-441 - Anti-dimethyl-Histone H3 (Lys9) Antibody

07-441 Sigma-Aldrich

Anti-dimethyl-Histone H3 (Lys9) Antibody

clone, Upstate®, from rabbit

Synonym: H3K9me2, Histone H3 (di methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, DE-DL, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   dot blot: suitable
  immunocytochemistry: suitable
  inhibition assay: suitable (peptide)
  western blot: suitable
species reactivity   human, human, mouse, rat, chicken, Schizosaccharomyces pombe
shipped in   dry ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_003493
UniProt accession no.   Q16695


General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.


Expected to cross react with mouse, chicken, rat and fission yeast, but not budding yeast. Broad species cross-reactivity expected.


KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..Rme2KSTG.., in which me2K corresponds to dimethyl-lysine at residue 9 of human Histone H3.


Dot Blot Analysis:
Specificity confirmed by an independent laboratory.

Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of Histone H3 in western blot analysis of HeLa acid extracts (Figure A, Lane 4).

Reported by an independent laboratory.

Use Anti-dimethyl-Histone H3 (Lys9) Antibody (Rabbit Polyclonal Antibody) validated in DB, ICC, PIA, WB to detect dimethyl-Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di methyl K9).

Target description

17 kDa

Physical form

Format: Purified

Storage and Stability

1 year at -20°C from date of shipment.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/ thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.


Routinely evaluated by Western Blot on HeLa acid extract and recombinant Histone H3 (cat. # 14-411).

Western Blot Analysis:
1:500 dilution of this lot detected dimethyl Histone H3 on 10 μg of HeLa acid extract but not on recombinant Histone H3.

Analysis Note

HeLa whole cell lysate, U2OS cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


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