• USA Home
  • 07-449 - Anti-trimethyl-Histone H3 (Lys27) Antibody

EMAIL THIS PAGE TO A FRIEND
07-449 Sigma-Aldrich

Anti-trimethyl-Histone H3 (Lys27) Antibody

Anti-trimethyl-Histone H3 (Lys27), also known as Anti-H3K27me3, is a highly published Rabbit Polyclonal Antibody. This protein A purified antibody is dot blot tested for trimethylated lysine 27 specificity and validated in WB, ICC, IP.

Synonym: H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3k27me3

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (07-449-AF488)
  200 µg (07-449-AF488)
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  multiplexing: suitable
  western blot: suitable
biological source   rabbit
clone   polyclonal
isotype   IgG
mfr. no.   Upstate
shipped in   dry ice
species reactivity   mouse, human
gene symbol   HIST1H3A(8350)
  HIST1H3B(8358)
  HIST1H3C(8352)
  HIST1H3D(8351)
  HIST1H3E(8353)
  HIST1H3F(8968)
  HIST1H3G(8355)
  HIST1H3H(8357)
  HIST1H3I(8354)
  HIST1H3J(8356)
  HIST3H3(8290)
Species Reactivity Notes   Human, mouse. Broad species cross-reactivity expected.
gene symbol   H3FA
  H3FB
  H3FC
  H3FD
  H3FF
  H3FH
  H3FI
  H3FJ
  H3FK
  H3FL
  H3FT
  H3T
  MGC126886

Description

Target description

17 kDa

Application

Immunocytochemistry:

Immunoprecipitation:

Beadlyte Histone-Peptide Specificity Assay:

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(me3K)SAP… in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Routinely evaluated by western blot in acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.4 µg/mL of anti-trimethyl histone H3 Lys27 was used on recombinant Histone H3 and HeLa cell acid extracts.

Specificity

Trimethylated histone H3 (Lys27). Broad species cross-reactivity expected.

Physical form

Format: Purified

Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide before the addition of 30% glycerol.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?