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  • 07-690 - Anti-Histone H3 Antibody, CT, pan

07-690 Sigma-Aldrich

Anti-Histone H3 Antibody, CT, pan

Use Anti-Histone H3 Antibody, CT, pan (rabbit polyclonal antibody) validated in ChIP, WB to detect Histone H3 also known as H3, Histone H3, H3 histone family member T, histone 3 H3, histone cluster 3 H3.

Synonym: H3, Histone H3, H3 histone family, member T, histone 3, H3, histone cluster 3, H3

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, HF-HI, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   ChIP: suitable
  western blot: suitable
species reactivity   human, human, chicken, mouse, rat, Saccharomyces cerevisiae, yeast, mouse, yeast, chicken, chicken, rat, human, rat, mouse
shipped in   dry ice
antibody form   serum
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_002107.3
UniProt accession no.   Q16695


General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.


Broad species cross-reactivity expected due to sequence homology.

Recognizes C-Terminal region of Histone H3, Mr 17 kDa


KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.


Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.

Target description

Approx. 17 kDa

Physical form

Rabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%.


Routinely evaluated by immunoblot on acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


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