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07-741 Sigma-Aldrich

Anti-AS160 (Rab-GAP) Antibody

Detect AS160 (Rab-GAP) using this Anti-AS160 (Rab-GAP) Antibody validated for use in WB.

Synonym: Acrg embryonic lethality minimal region ortholog, Akt substrate of 160 kDa, TBC (Tre-2, BUB2, CDC16) domain-containing protein, TBC1 domain family, member

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories AS-ATG, Alphabetical Index, Antibodies, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   mouse, rat, canine, human, human, mouse
shipped in   wet ice
antibody form   serum
isotype   IgG
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NP_055647
UniProt accession no.   O60343

Description

General description

AS160 (Akt Substrate of 160 kDa, Akt1S1), Rab GAP (GTPase activating protein), is phosphorylated on multiple sites by Akt in response to insulin in adipocytes and contraction in muscles. AS160 is the long sought after link between the upstream insulin signaling through insulin/PI3K/Akt and GLUT4 vessicle translocation to the cell membrane. It has 6 Akt phosphorylation consensus sequences that are believed to become phosphorylated upon insulin stimulation. This phosphorylation is required for translocation of GLUT4-containing vesicles to the cell membrane1. A mutation of two or more of these phosphorylation sites results in a dramatic decrease in AS160 activity and insulin-stimulated GLUT4 redistribution at the cell membrane2. AS160 has also shown to be important for GLUT4 expcytosis2.

Specificity

Predicted to cross react with rat and dog based on sequence homology.

Recognizes AS160 (Rab-GAP), Mr 160 kDa.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 1178-1189 (C-PTNDKAKAGNKP) of rat Akt substrate of 160kDa (AS160) (Rab-GAP) with a N-terminal cysteine for conjugation purposes.

Application

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Target description

Approx. 160 kDa

Physical form

Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and 30% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot in RIPA lysates from 3T3/L1 cells differentiated into adipocytes.

Western Blot Analysis:
A 1:1000-1:5000 dilution of this lot detected AS160 (Rab-GAP) in RIPA lysates from 3T3/L1 cells differentiated into adipocytes by MDI treatment. This lot also detects AS160 (Rab-GAP) in HEK293 lysates.

Analysis Note

Control
MDI-treated 3T3/L1 cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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