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1.00427 Millipore

Listeria agar (base) acc. OTTAVIANI and AGOSTI

acc. ISO 11290 Chromocult®

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Properties

form   medium granules (dehydrated (DCM))
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   7.0-7.4 (25 °C, 70 g/L in H2O)
solubility   70 g/L
Featured Industry   Food and Beverages
suitability   Listeria spp.
storage conditions   Store at +15°C to +25°C.

Description

Analysis Note

Appearance (colour): yellowish
Appearance (clearness): slightly opalescent to opalescent
pH-value (25 °C): 7.0 - 7.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Colony count (Listeria monocytogenes ATCC 13932 (WDCM 00021)):
Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 50 %
Colonies (Listeria monocytogenes ATCC 13932 (WDCM 00021)): blue green colonies with opaque halo
Inoculum on reference medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Colony count (Listeria monocytogenes ATCC 35152 (WDCM 00109)):
Recovery on test medium (Listeria monocytogenes ATCC 35152 (WDCM 00109)): ≥ 50 %
Colonies(Listeria monocytogenes ATCC 35152 (WDCM 00109)): blue green colonies with opaque halo
Growth (Listeria innocua ATCC 33090 (WDCM 00017)): no limit
Colonies (Listeria innocua ATCC 33090 (WDCM 00017)): blue green colonies without opaque halo
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): total inhibition
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): total inhibition
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Incubation: 44 ± 4 hours at 37 ± 1 °C
Reference media: Tryptic Soy Agar
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Detection of Listeria monocytogenes in Food and Environmental Samples

20-minute quick learning: detection of Listeria monocytogenes according to international standards
Keywords: Safety industry

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

The International Organization for Standardization (ISO), published revised EN ISO 11290-1 and EN ISO 11290-2 standards in 2017. In Part 1, a horizontal method for the detection of Listeria monocytog...
Keywords: Antibiotics, Culture media, Environmental, Microbiology

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