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1.00908 Millipore

Readybag® Buffered Peptone Water

According to ISO 6579, ISO 21528, ISO 22964, FDA-BAM, APHA, and EP; For microbiology

Synonym: Tryptone Phosphate Water, Peptone Water (buffered), BPW



Agency/Method   APHA
  ISO 21528
  ISO 22964
  ISO 6579
Quality Level   100
reg. compliance   FDA BAM
sterility   sterile; γ-irradiated
form   medium granules (dehydrated (DCM))
mfr. no.   READYBAG®
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   7.2 (37 °C, 25 g/L in H2O, after autoclaving)
solubility   25.5 g/L
bulk density   800 kg/m3
Featured Industry   Food and Beverages
suitability   Cronobacter spp.
  Salmonella spp.
storage conditions   Store at +15°C to +25°C.


General description

Readybag® Buffered Peptone Water is suitable for the preliminary non-selective enrichment of pathogenic Enterobacteriaceae. The broth is rich in nutrients and produces high resuscitation rates for sub-lethally injured bacteria and intense growth. The phosphate buffer system prevents bacterial damage caused by changes in the pH of the medium. Peptone, including an enzymatic digest of casein, acts as a source of carbon, nitrogen, vitamins, and minerals, while sodium chloride maintains the osmotic balance.


Readybag® Buffered Peptone Water is suitable for the preliminary non-selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae, such as Salmonella and Cronobacter, from food and animal feed, water, and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
Protocols & Articles


Ask the Expert: How to Choose the Right Method for Microbial Testing in Infant Formula?

Infant formula and their ingredients are very sensitive foods which must be carefully microbiologically analyzed before release to market. Recent recalls of infant formula products due to microbial c...
Dr. Andreas Bubert
Senior Global Product Manager Culture Media F&B
Keywords: AGE, Culture media, Diseases, Environmental, Food & Beverage, Food Safety, Microbiology, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sample preparations, Separation

New EN ISO 22964 for Cronobacter spp. detection in the food chain

The International Organization for Standardization (ISO) has published the new EN ISO 22964:2017 standard, which specifies a horizontal method for the detection of Cronobacter spp. in food, animal fe...
Keywords: Culture media, Environmental, Microbiology

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