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1.01597 Sigma-Aldrich

TB-Fluor phenol-free

Staining kit for the acid-fast smear examination with fluorescence microscopy (Auramin-Rhodamine staining)

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Properties

Related Categories Antibodies, Cell Biology, Hematology and Histology, Stains and Dyes
packaging   1 PC in Fibre case (1015970007)
  1 set in Fibre case (1015970001)
shipped in   ambient
storage temp.   Store at +15°C to +25°C.
specifications   Suitability for microscopy: passes test
  Mycobacteria: red-orange/yellow-green
  Background: dark

Description

Other Notes

Fluorescence staining allows acid-resistant Mycobacteria to be easily identified even when their numbers are small. As it has become an established procedure alongside Ziehl-Neelson staining (Tb-color modified), we provide three ready-to-use solutions in our TB-fluor, phenol-free staining kit. Of these, the first solution contains the two fluorescent dyes, Rhodamine B – staining red - and Auramine O – staining yellowish-green – which can be used depending on the filter combination of the microscope. Here, the auramine-rhodamine staining solution is prepared without phenol and the efficient penetration of the dyes into the target structures is achieved through a phenol substitute that is more user- and eco-friendly than phenol. Further, the kit supplies a decolorization solution and a potassium permanganate solution that turns the background uniformly black, suppressing troublesome intrinsic fluorescence of the specimen. If required, double staining with Tb-color modified (ZN) is possible. The Tb-fluor, phenol-free-kit, modified is designed to be used for smears, thougt it can also be used for paraffin sections.The kit is sufficient for up to 60-65 preparations and is an IVD / CE registered product.

Safety & Documentation

Safety Information

HS Code 
3822 00 00
Precautionary Statement(s) 
P210: Keep away from heat/sparks/open flames/hot surfaces. No smoking.
P240: Ground/bond container and receiving equipment.
P273: Avoid release to the environment.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P403 + P235: Store in a well-ventilated place. Keep cool.
Signal Word 
Danger
Storage class 
3 Flammable Liquids
Hazard Pictogram(s) 
GHS02  GHS02
Hazard Pictogram(s) 
GHS07  GHS07
Hazard Pictogram(s) 
GHS09  GHS09
Hazard Symbols 
Irritant  Irritant
Declaration (railroad and road) ADR, RID 
UN 3316 Entzündbarer flüssiger Stoff, ätzend, n.a.g.(SALZSAEURE UND ENTH. ISOPROPANOL), 9, II
Declaration (transport by air) IATA-DGR 
UN 3316 FLAMMABLE LIQUID, CORROSIVE, N.O.S.(HYDROCHLORIC ACID AND CONT. ISOPROPANOL), 9, II
Declaration (transport by sea) IMDG-Code 
UN 3316 FLAMMABLE LIQUID, CORROSIVE, N.O.S.(HYDROCHLORIC ACID AND CONT. ISOPROPANOL), 9, II
Categories of danger 
flammable
R Phrase 
R 10
Flammable.
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