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1.01697 SAFC

Benzonase® endonuclease

Emprove® Expert

Synonym: Endonuclease from Serratia marcescens



Related Categories Biopharmaceutical Manufacturing, Biopharmaceutical Process Development and Manufacturing, Cleavage and Lysis Enzymes for Biopharmaceutical Manufacturing More...
Quality Level   500
description   suitable for biopharmaceutical production EMPROVE® bio
product line   Emprove® Expert
pH   8.0 (25 °C in H2O)
storage conditions   Store at -20°C. Transport with dry ice.


General description

Benzonase® endonuclease acts as an unspecific endonuclease, degrading both DNA and RNA to small 3–5 base pairs (< 6 kDa) fragments with no base preference. It is the ideal tool for nucleic acid removal in virus vector and vaccine manufacturing as demanded by regulators. Additionally, the use of Benzonase® endonuclease increases the yield in virus purification, protects downstream chromatography and filter devices from fouling, and reduces feed stream viscosity.

Benzonase® endonuclease is the smart solution for DNA removal in viral vaccine and viral vector production and has proven its value for over 30 years. Balancing efficiency and regulatory compliance by delivering reliability and high-quality due manufacturing under good manufacturing practices (GMP ICH Q7). Our extensive documentation packages of Emprove® dossiers and the availability of a FDA Drug Master File type II, supports you in filing your vaccine manufacturing process to the authorities.


Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Analysis Note

Appearance (description): clear
Appearance (color): colourless
Activity (DNA; pH 8.0; 30 min; 37 °C): ≥ 250 U/µl
Spec. activity (calc. on protein): ≥ 1.1E+06 U/mg
Identity (electrophoresis): passes test
Purity (calc. on protein): ≥ 99.0%
Proteases: not detectable
Colony count (aerobic bacteria): <10 CFU in 100 000 U
Colony count (Yeasts and moulds): <10 CFU in 100 000 U
Endotoxines (LAL-test): <0.25 EU in 1000 U

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Emprove® Dossiers

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Protocols & Articles

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1.01681 Benzonase® ELISA kit II, suitable for detection
1.01695 Benzonase®, suitable for biopharmaceutical production EMPROVE® bio

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