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1.01865 Millipore

Readybag® Half FRASER (Demi FRASER) broth with supplements

acc. ISO 11290, 62 g, irradiated Readybag®

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Properties

Agency/Method   APHA
  ISO 11133
  ISO 11290
Quality Level   100
form   medium granules (dehydrated (DCM))
mfr. no.   READYBAG®
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   7.0-7.4 (25 °C, 55 g/L in H2O)
solubility   55 g/L
Featured Industry   Food and Beverages
suitability   Listeria spp.
storage conditions   Store at +15°C to +25°C.

Description

General description

Half FRASER (Demi FRASER) broth has high nutrient content and the large buffer capacity that creates optimum growth conditions for Listeria. Lithium chloride, nalidixic acid, and acriflavine hydrochloride largely inhibit the growth of accompanying bacteria. Esculin and ammonium iron (III) citrate enables the detection of the β-D-glucosidase activity by the formation of a black complex of esculetin with iron (III) ions. However, this reaction is not exclusive to Listeria sp. and hence every primary and secondary enrichment in FRASER broth has to be sub-cultured on selective plating media.

Application

Half FRASER (Demi FRASER) broth with supplements is used for the primary selective enrichment of Listeria sp. from food and animal feed as well as from environmental samples and other materials.

Legal Information

READYBAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Standards for Detection and Enumeration of Listeria monocytogenes and Listeria spp. in the food chain

The International Organization for Standardization (ISO), published revised EN ISO 11290-1 and EN ISO 11290-2 standards in 2017. In Part 1, a horizontal method for the detection of Listeria monocytog...
Keywords: Antibiotics, Culture media, Environmental, Microbiology

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