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1.02136 Millipore

Contact C

for coliforms, for Escherichia coli, ENVIROCHECK

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Properties

Related Categories Media by Application, Media for Cosmetic Industry, Microbiological Culture Media, Microbiology
Quality Level   100
form   solid (ready-to-use)
mfr. no.   ENVIROCHECK
application(s)   microbiology: suitable
Featured Industry   Food and Beverages
suitability   Escherichia coli (total count)
  coliforms
storage conditions   Store below +15°C.

Description

General description

(total coliforms/E.coli) Envirocheck

Envirocheck Contact Total Coliforms/E.Coli, a semi-quantitative detection of coliform bacteria on surfaces and in liquids, are flexible paddles that are coated with Plate Count Agar for simultaneous detection of coliforms and E. coli.Slide A is coated with Plate Count Agar to obtain the total viable (aerobic) count. Slide B is coated with Chromocult Coliform Agar for the simultaneous detection of coliforms and E. coli in water and food-samples.

Application

This medium is recommended for the simultaneous detection of coliforms and E. coli in water and food-samples. Envirocheck contact slides can be used for both surface and liquid testing in industrial and environmental monitoring.

Analysis Note

Count the number of colonies on both sides of the paddle. Plate Count Agar: Count the number of colonies for obtaining the total aerobic bacterial count (TVC). Chromocult Coliform Agar: Count the number of dark-blue to violet colonies (E. coli) and the number of salmon to red colonies for other coliforms. Additionally, E. coli can be confirmed by adding KOVAC′s Indole Reagent directly onto the colony (positive indole reaction: red colour).

Sterility: passes test
pH-value/side one (25 °C): 6.7 - 7.3
pH-value/side two (25 °C): 6.5 - 7.1
Growth/side one
- Escherichia coli ATCC 11775 (WDCM 00090):
good
- Escherichia coli O157:H7 ATCC 35150: good
- Citrobacter freundii ATCC 8090: good
- Salmonella enteritidis ATCC 13076 (WDCM 00030): good
Growth/side two
- Escherichia coli ATCC 11775 (WDCM 00090):
good
- Escherichia coli O157:H7 ATCC 35150: fair/good
- Citrobacter freundii ATCC 8090: good
- Salmonella enteritidis ATCC 13076 (WDCM 00030): good
Colonies
- Escherichia coli ATCC 11775 (WDCM 00090):
dark blue to violet
- Escherichia coli O157:H7 ATCC 35150: salmon to red
- Citrobacter freundii ATCC 8090: salmon to red
- Salmonella enteritidis ATCC 13076 (WDCM 00030): colourless
Incubation: 48 hrs.; 35 °C; aerobic.

Legal Information

ENVIROCHECK is a trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Protocols & Articles

Articles

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