1.02896 Millipore


Deoxycholate citrate agar acc. to LEIFSON, modified for microbiology



Quality Level   100
form   medium granules (dehydrated (DCM))
mfr. no.   GRANUCULT®
application(s)   microbiological culture: suitable
  microbiology: suitable
pH   7.4-7.6 (37 °C, 48 g/L in H2O, after autoclaving)
solubility   47.5 g/L
bulk density   690 kg/m3
Featured Industry   Food and Beverages
suitability   Salmonella spp.
  Shigella spp.
storage conditions   Store at +15°C to +25°C.


Analysis Note

Appearance (clearness): clear
Appearance (colour): reddish-brown
pH-value (25 °C): 7.3 - 7.7
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): poor to fair
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Growth (Shigella flexneri ATCC 12022 (WDCM 00126)): fair to very good
Growth (Shigella sonnei ATCC 11060): fair to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis NCTC 5188 (WDCM 00119)): good to very good
Growth (Proteus mirabilis ATCC 14273): good to very good
Growth (Staphylococcus aureus ATCC 25923 (WDCM 00034)): none
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): none
Colony colour (Escherichia coli ATCC 25922 (WDCM 00013)): pink-red
Colony colour (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): pink-red
Colony colour (Shigella flexneri ATCC 12022 (WDCM 00126)): colourless
Colony colour (Shigella sonnei ATCC 11060): colourless
Colony colour (Salmonella typhimurium ATCC 14028 (WDCM 00031)): colourless
Colony colour (Salmonella enteritidis NCTC 5188 (WDCM 00119)): colourless
Colony colour (Proteus mirabilis ATCC 14273): colourless to brownish
Black centre (Salmonella typhimurium ATCC 14028 (WDCM 00031)): +
Black centre (Salmonella enteritidis NCTC 5188 (WDCM 00119)): +
Black centre (Proteus mirabilis ATCC 14273): +/-
Incubation: 24 hrs.; 35 °C; aerobic.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Cultivation of Shigella

The genus Shigella consists of four species: S. dysenteriae, S. flexneri, S. boydii, and S. sonnei. These gram-negative, non-motile and non-sporulating bacilli belong to the Enterobacteriaceae family...
Keywords: AGE, Anaerobic, Culture media, Nucleic acid hybridization

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